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Image Search Results
Journal: Nature Communications
Article Title: Pretreatment with IL-15 and IL-18 rescues natural killer cells from granzyme B-mediated apoptosis after cryopreservation
doi: 10.1038/s41467-024-47574-0
Figure Lengend Snippet: a NK cell recovery immediately before and after cryopreservation ( n = 4 healthy donors). b Incucyte live imagining using caspase-3/7 cleavable dye to indicate early apoptosis ( n = 4 healthy donors). c Incucyte live imagining using Cytox NIR dye to indicate loss of plasma membrane integrity ( n = 4 healthy donors. d granzyme B levels at time 0 and 24 h after thawing ( n = 5 healthy donors). e GZMB knockout NK cells recovery after cryopreservation ( n = 4 healthy donors). f Perforin knockout NK cells recovery after cryopreservation ( n = 4 healthy donors). g , h GZMB and mock KO NK cells were cocultured at 1 ratio of 1:10 in both directions to determine the role of fratricide in NK cell death ( n = 4 healthy donors). i , j Confocal microscopy imaging showing the extent of colocalization of GZMB and LAMP-3, a marker for cytotoxic granules in fresh versus cryopreserved NK cells (fresh n = 29, cryopreserved n = 42). Normality test was used to determine the distribution of the data then parametric test t test was used for ( a , d , e , f , h ). nonparametric Mann–Whitney test was used for ( i ). A two-tailed test was used for ( a , d , e , f , h , i ). All graphs are shown as mean ± SEM.
Article Snippet: Fig. 2 GZMB leakage induces apoptosis in NK cells after cryopreservation. a NK cell recovery immediately before and after cryopreservation ( n = 4 healthy donors). b Incucyte live imagining using caspase-3/7 cleavable dye to indicate early apoptosis ( n = 4 healthy donors). c Incucyte live imagining using
Techniques: Membrane, Knock-Out, Confocal Microscopy, Imaging, Marker, MANN-WHITNEY, Two Tailed Test